Recipe

Protein concentration gel equilibration buffer

Mix the following components:

12.5-ml 0.5 M Tris-Cl (pH 6.8)

20-ml 10% SDS

5-ml 2-mercaptoethanol

10-ml glycerol

After mixing, add a few grains of bromophenol blue.

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  1. doi:10.1101/pdb.rec10665 Cold Spring Harb Protoc 2006: pdb.rec10665-
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