Table 1. Stains for the detection of total protein on a membrane
| Stain | Detection limit | Nitrocellulose | PVDF | Comments | Compatible with immunoblotting |
| Ponceau S | 1 μg | + | + | Reversible | Yes |
| Coomassie Blue R250 | 1 μg | + | + | Permanenthigh background | Yes |
| India Ink | 100 ng | + | + | Permanent | — |
| Colloidal Gold | 3 ng | + | + | Permanent | No |










