Table 1. Typical MuDPIT chromatography run setup

Step 1:	70-min gradient from 0% to 80% solvent B
	10 min held at 80% solvent B
Steps 2-13:	Each of these steps follows the sequence:
	5 min of 100% solvent A
	2 min of x% solvent C^a
	3 min of 100% solvent A
	10-min gradient from 0% to 10% solvent B
	90-min gradient from 10% to 45% solvent B
Step 14:	5-min 100% solvent A wash
	20-min 100% solvent C wash
	5-min 100% solvent A wash
	10-min gradient from 0% to 10% solvent B
	90-min gradient from 10% to 45% solvent B
Step 15:	5-min 100% solvent A wash
	20-min 100% solvent D wash
	5-min 100% solvent A wash
	10-min gradient from 0% to 10% solvent B
	90-min gradient from 10% to 45% solvent B
^aThe 2-minute solvent C percentages (x) in Steps 2-13 are as follows: 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 90%, 100%, and 100%, respectively.