| Step |
IPG length (cm) |
Voltage |
Duration (hr:min) |
Volt-hours |
Gradient type |
| 1 |
7, 11, 13, 18, 24 |
rehydration |
12:00a |
- |
- |
| 2 |
7 |
500 |
0:30 |
250 |
step and hold |
| 2 |
11, 13, 18, 24 |
200 |
1:00 |
500 |
step and hold |
| 3 |
7 |
8000b |
1:00 |
8000 |
step and hold |
| 3 |
11, 13 |
8000b |
2:00 |
16,000 |
step and hold |
| 3 |
18 |
8000b |
4:00 |
32,000 |
step and hold |
| 3 |
24 |
8000b |
6:25-11:25 |
50,000-90,000 |
step and hold |
| aThe total rehydration time can be varied for convenience, but it must be >6 hours.
|
| bDepending on the sample, this voltage may not be reached within the suggested step duration. For this reason, it is best to
program the steps based on volt-hours rather than time.
|
