Recipe

5X SDS-loading buffer

300 mM Tris-HCl (pH 8.0)

10% SDS

20 mM EDTA

25% ß-mercaptoethanol

0.1% bromophenol blue

50% glycerol

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  1. doi:10.1101/pdb.rec10700 Cold Spring Harb Protoc 2006: pdb.rec10700-
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