Imaging Microglia in Live Brain Slices and Slice Cultures
This protocol was adapted from “A Practical Guide: Imaging Microglia in Live Brain Slices and Slice Cultures,” Chapter 55, in Imaging in Neuroscience and Development (eds. Yuste and Konnerth). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2005.INTRODUCTION
This protocol describes methods for fluorescence labeling and time-lapse confocal imaging of microglia in acutely prepared tissue slices from developing and adult animals, and to slice cultures derived from early post-natal day 7 (<PND7) animals. The techniques permit real-time analyses of microglial structure and dynamic remodeling in live rodent brain tissues.
