Sectioning Mouse Embryos after Whole-Mount In Situ Hybridization
This protocol was adapted from “Techniques for Visualizing Gene Products, Cells, Tissues, and Organ Systems,” Chapter 16, in Manipulating the Mouse Embryo, 3rd edition, by Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.INTRODUCTION
Embryos that have been processed for whole-mount in situ hybridization are sectioned frozen or embedded in wax to visualize which tissues express the gene transcripts.
