Recipe

Fixing solution

2.25 mL acetic acid, glacial

0.25 mL formaldehyde stock solution (37%)

2.5 mL H2O

It is important to prepare fresh fixing solution every 2-3 h.

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  1. doi:10.1101/pdb.rec10912 Cold Spring Harb Protoc 2007: pdb.rec10912-
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