Microreagent B
4.0 g sodium bicinchoninic acid (BCA)
Dissolve the BCA in 100 mL of H2O. Although the disodium salt of BCA is soluble at neutral pH, the free acid is not readily soluble even at basic pH. Microreagent B is stable for 1-3 wk when stored in a plastic container at room temperature, and indefinitely at 4ºC.
