Recipe

GPI stain

Reagent Amount to add
20 mg/mL fructose-6-phosphate 1 mL
Glucose-6-phosphate dehydrogenase (Sigma G8289) 10 μL
0.2% (w/v) MgCl2 9 mL
2.7 mg/mL NADP 1 mL
2.7 mg/mL 4-Nitro blue tetrazolium chloride (NBT) 1 mL
Tris-citrate buffer (pH 8.0) 1 mL
10 mg/mL pregnant mare serum (PMS) 30 μL
Freshly prepare the stain. Mix all reagents, adding PMS at the last minute.

| Table of Contents

This Article

  1. doi:10.1101/pdb.rec11094 Cold Spring Harb Protoc 2007: pdb.rec11094-
  1. » Full Text

Article Category

  1. Recipe

Personal Folder

  1. Save to Personal Folders

Updates/Comments

  1. Submit Updates/Comments
  2. No Updates/Comments published
  3. Alert me when Updates/Comments are published

Share

Current Issue

  1. January 2026, 2026 (1)
  1. Current Issue