Recipe

Hydroxyl-radical footprinting binding buffer

20 mM HEPES-KOH (pH 7.5)

50 mM KCl

5 mM MgCl2

100 μg/mL BSA

5 μg/mL poly(dI-dC)

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  1. doi:10.1101/pdb.rec11119 Cold Spring Harb Protoc 2007: pdb.rec11119-
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