Recipe

Detergent buffer

25 mM Tris-HCl (pH 8)

0.2 M NaCl

1% NP-40

1% deoxycholate

1 mM EDTA

To inhibit proteolysis, add the following protease inhibitors just before use:

1 mM PMSF

5 μg/ml pepstatin A

5 μg/ml leupeptin

5 μg/ml aprotinin

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  1. doi:10.1101/pdb.rec10831 Cold Spring Harb Protoc 2007: pdb.rec10831-
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