Recipe

Extraction buffer

8 M urea

Use ultrapure urea (e.g., ICN Biomedicals). Urea solutions should be freshly deionized using analytical-grade mixed bed resin, AG 501-X8 (D) from Bio-Rad.

50 mM Tris-HCl (pH 7.4)

5 mM EGTA

0.2% 2-mercaptoethanol

1 mM phenylmethylsulfonyl fluoride (PMSF)

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  1. doi:10.1101/pdb.rec10832 Cold Spring Harb Protoc 2007: pdb.rec10832-
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