Recipe

Homogenization buffer I

50 mM Tris-HCl (pH 7.4)

5 mM MgCl2

0.1% 2-mercaptoethanol

1 mM phenylmethylsulfonyl fluoride (PMSF)

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  1. doi:10.1101/pdb.rec10833 Cold Spring Harb Protoc 2007: pdb.rec10833-
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