TNE

10 mM Tris-HCl (pH 8)

100 mM NaCl

1 mM EDTA

For use in purification of intermediate filament protein from bacterial inclusion bodies, to inhibit proteolysis, add the following protease inhibitors just before use:

1 mM PMSF

5 μg/ml pepstatin A

5 μg/ml leupeptin

5 μg/ml aprotinin