Recipe

GUS extraction buffer

10 mM EDTA (pH 8.0)

0.1% SDS

50 mM sodium phosphate (pH 7.0)

0.1% Triton X-100

Keep at room temperature.

If performing assays on tissue extracts, just before use, add:

10 mM ß-mercaptoethanol

25 μg/ml PMSF

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  1. doi:10.1101/pdb.rec10859 Cold Spring Harb Protoc 2007: pdb.rec10859-
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