Recipe

2X SDS-PAGE gel-loading buffer

2.5% (w/v) SDS

25% glycerol

125 mM Tris-Cl (pH 6.8)

0.01% (w/v) bromophenol blue

Immediately before use add:

100 mM fresh dithiothreitol (DTT)

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  1. doi:10.1101/pdb.rec9191 Cold Spring Harb Protoc 2007: pdb.rec9191-
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