Recipe

PBS for FISH (20X, pH 7.4)

Reagent Amount to add (for 20X) Final concentration (1X)
NaCl 160 g 140 mM
KCl 4 g 2.7 mM
Na2HPO4•2 H2O 36 g 6.5 mM
KH2PO4 4.8 g 1.5 mM
H2O 1 liter
HCl (1 M) adjust pH to 7.4
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  1. doi:10.1101/pdb.rec10944 Cold Spring Harb Protoc 2007: pdb.rec10944-
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