Recipe

dNTP mix for NT

dATP (100 mM)

dCTP (100 mM)

dGTP (100 mM)

dTTP (100 mM)

Mix 5 μl of each of dATP, dCTP, and dGTP to 1 μl of dTTP and 984 μl of H2O (autoclaved). The final concentrations are 0.5 mM dATP, dCTP, and dGTP, and 0.1 mM dTTP. Store at −20°C.

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  1. doi:10.1101/pdb.rec10963 Cold Spring Harb Protoc 2007: pdb.rec10963-

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