Recipe

CD34+ transduction medium

Iscove’s modified Dulbecco’s medium (IMDM), supplemented with:

20% BIT9500 (Stem Cell Technology),

100 ng/ml of stem cell factor (SCF),

100 ng/ml of Flt3-ligand, and

10 ng/ml of thrombopoietin (TPO).

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  1. doi:10.1101/pdb.rec11003 Cold Spring Harb Protoc 2007: pdb.rec11003-
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