Recipe

Embedding resin

Mixture A
2-Dodecenylsuccinic acid anhydride (DDSA) 62 ml
Glycid ether 100 (Epon 812) 100 ml
Mixture B
Glycid ether 100 (Epon 812) 100 ml
Methyl nadic anhydride (MNA) 89 ml
Prepare mixtures A and B in separate beakers while stirring. The mixtures are stable for 6 months if stored at −20°C.
Combine 5 ml of mixture A, 5 ml of mixture B, and 0.15 ml of 2,4,6-tris(dimethylaminomethyl)phenol while stirring. This will yield blocks of medium hardness. The proportions of mixtures A and B can be varied according to the desired hardness of the blocks (for harder blocks, increase the amount of mixture B relative to the amount of mixture A).
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  1. doi:10.1101/pdb.rec11057 Cold Spring Harb Protoc 2007: pdb.rec11057-

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