Orange G gel-loading buffer
Ficoll
Orange G (acid orange 10)
To prepare a 10-mL solution, dissolve 1 g of Ficoll in 10 mL of H2O. Vortex and heat the solution at 37°C in a water bath until the Ficoll is well dissolved. Add a small spatula tip of Orange G to color the buffer. Filter-sterilize the solution and store it at 4°C or room temperature.
