Recipe

Nuclear preparation buffer (NPB) (2X)

2 mM dithiothreitol

2 mM EDTA (pH 8.0)

30 mM HEPES

1 mM spermidine trihydrochloride

0.4 mM spermine tetrahydrochloride

500 mM sucrose

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  1. doi:10.1101/pdb.rec11138 Cold Spring Harb Protoc 2007: pdb.rec11138-
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