Recipe

Sperm dilution buffer (SDB)

75 mM KCl

0.5 mM spermidine trihydrochloride

0.2 mM spermine tetrahydrochloride

250 mM sucrose

Add ~80 μL of 0.1 N NaOH per 20 mL of solution to titrate the pH to 7.3-7.5. Store 0.5-1 mL aliquots at -20°C.

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  1. doi:10.1101/pdb.rec11139 Cold Spring Harb Protoc 2007: pdb.rec11139-
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