Recipe

Sterile cortex buffer

Reagent Amount to add Final concentration
NaCl 7.21 g 125 mM
KCl 0.372 g 5 mM
Glucose 1.802 g 10 mM
HEPES 2.38 g 10 mM
1 M CaCl2 2 mL 2 mM
1 M MgSO4 2 mL 2 mM
H2O to 1 L --
Adjust pH to 7.4.

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  1. doi:10.1101/pdb.rec11198 Cold Spring Harb Protoc 2008: pdb.rec11198-
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