Recipe

AP buffer (Mg-free)

Reagent Quantity (for 100 mL) Final concentration
NaCl-Tris solution (5X) 20 mL 1X
Tween 20 (20%) 500 μL 0.1%
H2O to 100 mL
Keep for up to 1 wk. Filter (0.45 μm) before use.

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  1. doi:10.1101/pdb.rec11533 Cold Spring Harb Protoc 2008: pdb.rec11533-
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