Recipe

Nuclear digestion buffer

Reagent Quantity (for 10 mL) Final concentration
EDTA, 0.5 M (pH 8.0) 0.1 mL 5 mM
Proteinase K 1 mg 0.1 mg/mL
SDS (Sodium dodecyl sulfate) 0.1 g 1%
Tris-Cl, 1 M (pH 7.4) 1 mL 100 mM

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  1. doi:10.1101/pdb.rec11546 Cold Spring Harb Protoc 2008: pdb.rec11546-
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