Recipe

Nuclei buffer

Reagent Quantity (100 mL) Final concentration
EDTA, 0.5 M (pH 8.0) 0.1 mL 0.5 mM
Magnesium acetate, 1 M 0.5 mL 5 mM
Sucrose 5 g 5%
Tris-Cl, 1 M (pH 7.4) 2 mL 20 mM

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  1. doi:10.1101/pdb.rec11547 Cold Spring Harb Protoc 2008: pdb.rec11547-
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