Recipe

5X SDS sample buffer for HMTase assay

250 mM Tris-Cl (pH 6.8)

10% SDS

50% glycerol

0.2% bromophenol blue

Add 10% β-mercaptoethanol prior to use.

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  1. doi:10.1101/pdb.rec11281 Cold Spring Harb Protoc 2008: pdb.rec11281-
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