Recipe

2X SDS sample buffer for HMTase assay

100 mM Tris-Cl (pH 6.8)

4% SDS

20% glycerol

0.2% bromophenol blue

Add 10 % β-mercaptoethanol prior to use.

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  1. doi:10.1101/pdb.rec11282 Cold Spring Harb Protoc 2008: pdb.rec11282-
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