Table 1. Some mammalian antibodies that are effective for whole-mount immunocytochemistry in Xenopus embryos
| Antibody | Supplier and catalog number | Dilution |
|---|---|---|
| Anti-γ tubulin | Abcam ab11321 | 1:500 |
| Anti-γ tubulin | Abcam ab27076 | 1:500 |
| Centrin1 | Abcam ab11257 | 1:500 |
| Sec8 | Abcam ab13254 | 1:100 |
| α tubulin | Sigma T 9026 | 1:300 |
| α tubulin | Abcam ab4074 | 1:300 |
| Spectrin | Sigma S 1515 | 1:200 |
| ZO-1 | Invitrogen (Zymed) 61-7300 | 1:50 |
| PRC1 | Biolegend 603001 | 1:50 |
| Laminin receptor | Abcam ab2508 | 1:300 |
| Phospho-Smad | Cell Signaling Technology T 9511 | 1:100 |
