Recipe

DIP binding/wash buffer

Reagent Amount to add Final concentration
KCl 3.728 g 100 mM
MgCl2 203.31 mg 2 mM
1 M Tris-Cl (pH 7.4) 1 mL 2 mM
ZnSO4 1.43 mg 10 μM
Glycerol 50 mL 10%
H2O to 500 mL -

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  1. doi:10.1101/pdb.rec11310 Cold Spring Harb Protoc 2008: pdb.rec11310-
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