| Diagnostic PCR |
Overcome inhibition from meat, blood, and feces |
rTth (Applied Biosystems)
|
BSA, gp32, Tween 20, Betaine, PEG 400 |
Abu Al-Soud and Rådström 1998, 2000 |
|
Overcome inhibition from vitreous fluids |
Tth (Promega Corporation)
|
Triton X-100 |
Wiedbrauk et al. 1995 |
|
Increase sensitivity |
Tth (Roche Diagnostics)
|
--- |
Shames et al. 1995; Kim et al. 2001 |
|
Increase sensitivity |
rTth (Applied Biosystems)
|
--- |
Dahlenborg et al. 2001 |
|
Increase specificity and sensitivity |
Tth (MBI Fermentas)
|
W1 detergent (Gibco-BRL), BSA |
Laigret et al. 1996 |
|
Overcome inhibition from phenol
|
Tth (Amersham)
|
--- |
Katcher and Schwartz 1994 |
| Reverse Transcription-PCR |
Increase sensitivity |
rTth (Applied Biosystems)
|
--- |
Schwab et al. 2001 |
|
Reduce intra-PCR variability and increase sensitivity |
Pwo (Roche Diagnostics)
|
--- |
Mullan et al. 2001 |
|
Perform PCR of GC-rich sequences
|
3 different DNA polymerases |
Betaine |
Henke et al. 1997 |
| Cloning and sequencing |
Reduce amplification errors |
Pfu (Stratagene)
|
--- |
Cline et al. 1996; Shafikhani 2002 |
|
Reduce amplification errors
|
Pfu (Stratagene)
|
--- |
|
| Genotyping |
Improve amplification capacity |
DyNAzyme (Finnzymes) |
--- |
Zsolnai and Fesus 1997 |
|
Increase yield and specificity |
AmpliTaq Gold (Applied Biosystems)
|
--- |
Moretti et al. 1998 |
|
Reveal polymorphism |
DyNAzyme EXT (Finnzymes)
|
Betaine |
Diakou and Dovas 2001 |
| Real-time PCR |
Overcome inhibition from sludge |
AmpliTaqGold (Applied Biosystems)
|
Polyvinylpyrrolidone, gp32 |
Monpoeho et al. 2000 |
|
Overcome inhibition from buffered peptone water |
rTth (Applied Biosystems)
|
Glycerol |
Knutsson et al. 2002c |
|
Enable detection |
15 different DNA polymerases |
--- |
Kreuzer et al. 2000 |
|
Increase sensitivity |
Platinum Taq (Gibco-BRL)
|
--- |
Hein et al. 2001 |
