Recipe

Activation buffer

Reagent stock concentration Final concentration
1 M MES/KOH (pH 5.6), filter-sterilized 10 mM
1 M MgCl2, autoclaved 10 mM
150 mM Acetosyringone (3′,5′-Dimethoxy-4′-hydroxyacetophenone) in DMSO, stored at −20°C 150 μM

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  1. doi:10.1101/pdb.rec11332 Cold Spring Harb Protoc 2008: pdb.rec11332-
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