Recipe

Enzyme solution 1

55% (v/v) basic solution

0.25% bovine serum albumin, Fraction V (EMD/Calbiochem)

0.7% (w/v) cellulase (Cellulysin, Calbiochem)

45% H2O

0.1% (w/v) polyvinylpyrrolidone 40 (PVP 40)

Adjust pH to 5.5 with Tris.

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  1. doi:10.1101/pdb.rec11362 Cold Spring Harb Protoc 2008: pdb.rec11362-
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