Recipe

Enzyme solution 2

Basic solution

0.25% bovine serum albumin (w/v)

1.5% cellulase (w/v), “Onozuka” RS (Yakult Honsha Co.)

0.02% Pectolyase Y-23 (w/v) (Kyowa Chemical Products Co.)

Adjust the pH to 3.5 with HCl for 5 min to inactivate contaminating proteases.

Adjust the pH to 5.5 with KOH.

Filter through a 0.45-μm filter.

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  1. doi:10.1101/pdb.rec11364 Cold Spring Harb Protoc 2008: pdb.rec11364-
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