Recipe

NTMT for SISH

Reagent Amount to add (for 100 mL) Final concentration
3 M NaCl 3.33 mL 100 mM
1 M Tris-Cl (pH 9.6) 10 mL 100 mM
1 M MgCl2 5 mL 50 mM
10% Tween 20 1 mL 0.1% (w/v)
RO-H2O 80.67 mL
Prepare immediately before use.
RO-H2O is RNase-free H2O that has been purified by reverse osmosis (18 MΩ).
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  1. doi:10.1101/pdb.rec11411 Cold Spring Harb Protoc 2008: pdb.rec11411-
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