Recipe

IHC blocking buffer

Phosphate-buffered saline (PBS) for SISH

Sheep serum (heat-inactivated)

Prepare 1% (v/v) heat-inactivated sheep serum in PBS for SISH.

To prepare heat-inactivated serum, incubate stocks of sheep serum for 1 h at 56°C. Aliquot into smaller tubes and store at −20°C.

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  1. doi:10.1101/pdb.rec11416 Cold Spring Harb Protoc 2008: pdb.rec11416-

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