SSC (pH 5)
NaCl
RO-H2O (H2O that has been purified by reverse osmosis [18 MΩ])
To prepare a 20X stock solution, dissolve 175.3 g NaCl and 88.2 g sodium citrate•2H2O in 800 mL RO-H2O. Use citric acid to adjust the pH to 5. Adjust the volume to 1 L with RO-H2O. Autoclave and store at room temperature.
To make more dilute solutions of SSC, dilute the 20X stock with the appropriate volume of RO-H2O.
