Recipe

Coloration buffer

Reagent Amount to add Final concentration
1 M Tris-HCl, pH 9.5 5 mL 100 mM
1 M MgCl2 2.5 mL 50 mM
5 M NaCl 1 mL 100 mM
20% Tween 20 (Sigma-Aldrich P1379) 250 μL 0.1% (v/v)
H2O (sterile) to 50 mL
Combine reagents immediately before use. At room temperature, the solution eventually will precipitate.

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  1. doi:10.1101/pdb.rec11430 Cold Spring Harb Protoc 2008: pdb.rec11430-
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