Table 1. Sample data for a calibration curve for the BCA assay

Table

Sample number	Protein (μg)	Standard solution 2 mg/mL (μL)	Experiment buffer (μL)	BCA reagent (mL)	A₅₆₂
1	0	-	50	1	0
2	12.5	6.25	43.75	1	0.21
3	12.5	6.25	43.75	1	0.21
4	12.5	6.25	43.75	1	0.18
					Avg = 0.20

5	25.0	12.50	37.5	1	0.53
6	25.0	12.50	37.5	1	0.45
7	25.0	12.50	37.5	1	0.50
					Avg = 0.49

8	37.5	18.75	31.25	1	0.80
9	37.5	18.75	31.25	1	0.72
10	37.5	18.75	31.25	1	0.77
					Avg = 0.76

11	50.0	25.00	25.00	1	1.06
12	50.0	25.00	25.00	1	1.02
13	50.0	25.00	25.00	1	1.07
					Avg = 1.05

14	100.0	50.00	-	1	1.95
15	100.0	50.00	-	1	2.10
16	100.0	50.00	-	1	2.01
					Avg = 2.02

Adjust the concentration of the protein standard to 20 μg/mL by measuring the A_280nm or the A_260nm/A_280nm to determine the protein content, and dilute with sample buffer as necessary.