Recipe

Mannitol fusion medium

Mannitol (280 mM)

MgSO4 (0.1 mM)

HEPES (0.5 mM)

Bovine serum albumin (BSA) (0.05%, w/v)

Adjust pH to 7.0 and osmolarity to 255-260 mOsm. Filter medium with a 0.2-μm syringe filter.

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  1. doi:10.1101/pdb.rec11442 Cold Spring Harb Protoc 2008: pdb.rec11442-
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