Recipe

Gelatin solution (0.1%, w/v) for MEF

0.5 g gelatin (Type A, from porcine skin) (Sigma G1890)

500 mL H2O

Microwave on medium-high power for 2-4 min to dissolve the gelatin, and filter the solution with a 0.2-μm syringe filter. Alternatively, the solution can be autoclaved.

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  1. doi:10.1101/pdb.rec11444 Cold Spring Harb Protoc 2008: pdb.rec11444-

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