Recipe

Hypotonic solution for hESC

0.054 M KCl (4 g/L in H2O)

0.0136 M sodium citrate (4 g/L in H2O)

Mix the two solutions at a 1:1 ratio to bring the final concentrations to 0.027 M KCl and 0.0068 M sodium citrate.

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  1. doi:10.1101/pdb.rec11452 Cold Spring Harb Protoc 2008: pdb.rec11452-
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