hESC differentiation medium
400 mL Knockout DMEM (Invitrogen 10829-018)
100 mL fetal bovine serum
5 mL non-essential amino acids (10 mM; Invitrogen 11140-050)
5 mL L-glutamine (200 mM; Invitrogen 25030-081)
5 mL β-mercaptoethanol (100X; Chemicon)
Filter the medium with a 0.2-μm bottle filter.
