Recipe

CAM digestion buffer

Reagent Quantity (for 100 mL) Final concentration
EDTA (0.5 M, pH 8.0) 5 mL 25 mM
NaCl (5 M) 1 mL 50 mM
Proteinase K (20 mg/mL) 0.5 mL 0.1 mg/mL
SDS (10% w/v) 10 mL 1% w/v
Tris-Cl (1 M, pH 8.0) 1 mL 10 mM
H2O to 100 mL

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  1. doi:10.1101/pdb.rec11599 Cold Spring Harb Protoc 2009: pdb.rec11599-
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