Recipe

PBS for lamprey embryos (10X)

Reagent Quantity (for 1 L) Final concentration
NaH2PO4 2.75 g 23 mM
NaCl 90 g 1.54 M
Na2HPO4•7H2O 21.45 g 80 mM
H2O to 1 L
Adjust pH to 7.4 with NaOH. Store for several months at room temperature.

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  1. doi:10.1101/pdb.rec11603 Cold Spring Harb Protoc 2009: pdb.rec11603-
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