Recipe

Color development solution

Reagent Quantity (for 10 mL) Final concentration
NBT (4-Nitro blue tetrazolium chloride) (100 mg/mL) 45 μL 0.45 mg/mL
BCIP (5-Bromo-4-chloro-3-indolyl-phosphate) (50 mg/mL) 35 μL 0.175 mg/mL
NTMT(L) to 10 mL
Prepare just enough color development solution for the reaction. Excess can be stored for several days in foil-covered tubes at 4°C.

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  1. doi:10.1101/pdb.rec11606 Cold Spring Harb Protoc 2009: pdb.rec11606-
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