Recipe

NTMT(L)

Reagent Quantity (for 200 mL) Final concentration
MgCl2 (2 M) 5 mL 50 mM
NaCl (5 M) 4 mL 100 mM
Tris-Cl (1 M, pH 9.5) 20 mL 100 mM
Tween 20 200 μL 0.1%
H2O to 200 mL
There is no need to adjust the pH if a Tris-Cl stock solution is used. This solution should be prepared fresh before use. It can be stored for 12-24 h at room temperature. Precipitates form when stored for longer periods.

| Table of Contents

This Article

  1. doi:10.1101/pdb.rec11605 Cold Spring Harb Protoc 2009: pdb.rec11605-
  1. » Full Text

Article Category

  1. Recipe

Personal Folder

  1. Save to Personal Folders

Updates/Comments

  1. Submit Updates/Comments
  2. No Updates/Comments published
  3. Alert me when Updates/Comments are published

Share

Current Issue

  1. March 2026, 2026 (3)
  1. Current Issue