Recipe

KSOM (for RNAi)

95 mM NaCl

2.5 mM KCl

0.35 mM KH2PO4

0.2 mM MgSO4

10 mM sodium lactate

0.2 mM glucose

0.2 mM sodium pyruvate

25 mM sodium bicarbonate (NaHCO3)

1.71 mM CaCl2

1 mM L-glutamine

0.01 mM EDTA

1 mg/mL bovine serum albumin (BSA)

10 μg/mL gentamicin

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  1. doi:10.1101/pdb.rec11630 Cold Spring Harb Protoc 2009: pdb.rec11630-
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